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KMID : 0861020110260020051
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Korea Journal of Herbology
2011 Volume.26 No. 2 p.51 ~ p.57
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The anti-inflammatory effect of Achyranthes japonica on lipopolysaccharide-induced inflammatory activity in murine macrophages
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Kim Min-Sun
Jeong Jin-Soo
Lee Hye-Youn
Ju Young-Sung
Bae Gi-Sang
Seo Sang-Wan
Cho Il-Joo
Park Sung-Joo
Song Ho-Joon
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Abstract
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Objectives:Achyranthes japonica (AJ) has been used as an anti-bacterial and anti-inflammatory agent. However, it is unclear that AJ could show the anti-inflammatory effects in macrophages. In this experiment, we studied whether AJ could inhibit the inflammatory responses in macrophages.
Methods: To measure out the cytotoxicity of AJ, we performed the MTT assay. We evaluated the nitric oxide (NO) production, and cytokine production such as interleukin (IL)-1b, IL-6 and tumor necrosis factor (TNF)-a. We also investigated the cellular mechanims such as mitogen activated protein kinases (MAPK)s and nuclear factor kappa B (NF-kB).
Results: AJ inhibited lipopolysaccharide (LPS)-induced NO production. AJ also inhibited production levels of IL-1b, IL-6 and TNF-a in LPS-stimulated macrophage. Finally, western blot analysis showed that AJ treatment inhibited the activation of p38 but not of extracellular signal-regulated kinase, c-jun NH2-terminal kinase and NF-kB.
Conclusions :These results showed that AJ down-regulated the inflammatory response via p38 in macrophages, which suggest that AJ could be a candidate on treating inflammatory diseases.
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KEYWORD
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Achyranthes japonica (AJ), cytokine, inflammation
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